Our PEDD has been used to detect the HNE level in both tear and wound fluids of 9 patients, and the limitations of detection were about 0.76 μg/mL and 0.63 μg/mL respectively. Our device requires only 15 minutes, 3 μL of clinical sample for each test, and offers superior sensitivity compared to other current HNE detection methods. The HNE concentrations in the patient samples have shown that the elevated HNE levels might suggest an abnormal healing of either acute or chronic wounds, consistent with previous studies that showed excess protease levels in all non-healing wounds.
Above table compared our PEDD with other HNE detection methods or POC devices for wound assessment.
In conclusion, our PEDD provides potentially valuable insights on the relationships between wound status and HNE level. In the near future, we hope that our approach may be used to develop PEDD assays using different substrates and targeting additional biomarkers for clinical wound assessment as well as for the investigation of additional disease states.

Technology

S11101T0262

National Cheng Kung University(國立成功大學)

Academic institution

Prototype

2022/01/06

Joint development,

2024/01/06